SCIENCE-BOOK 29.04.2025 - Flipbook - Page 6
FILLERS
Safety pro昀椀le
Summary of the publication:
02
Safety of PEGylated Hyaluronic Acid Filler
for the Treatment of Facial Skin Aging: Case Report
Authors: Kubik P. et Gruszczyński W. I Published on 20.04.2024
INTRODUCTION
The aim of the case report was to evaluate the cellular and clinical response of a
therapeutic protocol aimed at countering facial skin aging.
A healthy 60-year-old female during one session underwent filler procedure to lift
and restore lack of volume of subcutaneous tissue with Neauvia Stimulate (Matex
Lab, Geneva, Switzerland). Skin biopsy was taken before the procedure from the
preauricular area, then on day 0, 7, 14 and 21 after the procedure. Histopathological
tests were to determine detailed tissue reactions that stand behind the results. The
patient underwent the following protocol: Day 0: Injection of 2.0 cc of Neauvia
Stimulate; malar area-dermal.
RESULTS
The patient who underwent the therapy for skin laxity, confirmed improvement of skin
elasticity by subjective measurements of skin elasticity, and detailed histopathological tests.
Day 0:
H&E staining revealed normal tissue structure, without inflammatory infiltration.
Masson’s Trichrome staining revealed single collagen fibers (0 - loose, regular).
CD34 – positive reaction.
Day 7:
H&E staining revealed numerous proliferating fibroblasts with large nuclei
and a small, slightly basophilic fragment of amorphous substance, which may
correspond to a fragment of hyaluronic acid.
Trichrome Masson staining revealed individual collagen fibers (0 - loose, regular).
Day 14:
H&E staining revealed inflammatory infiltrate (on a scale of 0–5: 2), located
mainly around the vessels, and proliferating fibroblasts.
Trichrome Masson staining revealed numerous collagen fibers (1 - loose, irregular).
Day 21:
H&E staining revealed a small inflammatory infiltrate (on a scale of 0–5:1).
Located mainly around the vessels. There is a decrease in the number
of proliferating fibroblasts compared to sections taken on day 14.
Trichrome Masson staining revealed numerous collagen fibers in the dermis (2 – dense).
Before vs. 7 days after procedure
15,9%
p value 0.2491
Before vs. 14 days after procedure
17,0%
p value 0.2294
Before vs. 21 days after procedure
27,3%
p value 0.0313
Table 1: Statistical analysis results for collagen fluorescence intensity
KEY MESSAGES
Based on our observations and other studies, it can be concluded that the PEG used
in these products (used as a cross-linking agent) has an immunomodulatory effect,
limiting the inflammatory response at the site of application.
The patient who underwent the therapy for skin laxity, confirmed improvement of skin
elasticity by subjective measurements of skin elasticity, and detailed histopathological tests.
A treatment with PEG-cross-linked hyaluronic acid leads to numerous positive
cutaneous changes after histological and immunological evaluations.
CONCLUSION
Based on our observations, we can conclude that the choice of fillers based on
hyaluronic acid cross-linked with polyethylene glycol should be considered as safe.
The evolution of inflammatory infiltrate may result from the immunomodulatory effect
of polyethylene glycol. This effect has a very positive impact on the safety profile of
the treatment itself and the context of using the hyaluronic acid filler itself, both in the
short and long term. The results of the presented case report seem to be interesting
and important from the point of view of the risks resulting from short and long-term
effect of the hyaluronic acid implant in the skin.
Reference : Kubik P, Gruszczyński W. Safety of PEGylated Hyaluronic Acid Filler for the Treatment of Facial Skin Aging: Case Report.
Clin Case Rep Int. 2024; 8: 1679.
Back to the safety profile page
